专业从事离子液体的合成、应用及其相关技术的研究工作----Lanzhou Greenchem ILs/青岛奥立科

题名

[Wiley数据库][2013] Determination of arecoline in areca nut based on field amplification in capillary electrophoresis coupled with electrochemiluminescence detection

参考引用方式

Qian Xiang, Ying Gao, Bingyan Han, Jing Li, Yunhong Xu and Jianyuan Yin. Luminescence 2013; 28: 50–55

研究机构

长春工程学院；中科院长春化学所

所涉及离子液体

1-丁基-3-甲基咪唑四氟硼酸盐                                           174501-65-6

研究领域

电化学发光；毛细管电泳；槟榔碱测定；离子液体

论文摘要

A sensitive capillary electrophoresis–electrochemiluminescence (CE–ECL) assay with an ionic liquid (IL) was developed for the determination of arecoline in areca nut. The IL, 1-butyl-3-methylimidazolium tetrafluoroborate (BMImBF₄), was an effective additive improved not only the separation selectivity but also the detection sensitivity of the analyte. BMImBF₄ in the separation electrolyte made the resistance of the separation buffer much lower than that of the sample solution, which resulted in an enhanced field amplified electrokinetic injection CE. ECL intensity of arecoline is about two times higher than that of the analyte with phosphate–IL buffer system. Resolution between arecoline and other unknown compounds in real samples was improved. Under the optimized conditions (ECL detection at 1.2 V, 16 kV separation voltage, 20 mmol/L phosphate with 10 mmol/L BMImBF₄ buffer at pH 7.50, 5 mmol/L Ru(bpy)₃ ²⁺ and 50 mmol/L phosphate buffer in the detection reservoir), a detection limit of 5×10^–9 mol/L for arecoline was obtained. Relative standard deviations of the ECL intensity and the migration time were 4.51% and 0.72% for arecoline. This method was successfully applied to determination of the amount of arecoline in areca nut within 450 s.

关键词

electrochemiluminescence; capillary electrophoresis; arecoline, ionic liquid