专业从事离子液体的合成、应用及其相关技术的研究工作----Lanzhou Greenchem ILs/青岛奥立科

题名

[Wiley数据库][2009] Ionic-Liquid-Doped Polyaniline Inverse Opals: Preparation, Characterization, and Application for the Electrochemical Impedance Immunoassay of Hepatitis B Surface Antigen

参考引用方式

Xing-Hua Li, Lin Dai, Yan Liu, Xiao-Jun Chen, Wei Yan, Li-Ping Jiang, and Jun-Jie Zhu. Adv. Funct. Mater. 2009, 19, 3120–3128.

研究机构

南京大学化工学院

所涉及离子液体

1-丁基-3-甲基咪唑四氟硼酸盐   174501-65-6

研究领域

离子液体掺杂聚苯胺；反蛋白石；电化学阻抗免疫分析

论文摘要

A 3D ordered macroporous (3DOM) ionic-liquid-doped polyaniline (IL-PANI) inverse opaline film is fabricated with an electropolymerization method and gold nanoparticles (AuNPs) are assembled on the film by electrostatic adsorption, which offers a promising basis for biomolecular immobilization due to its satisfactory chemical stability, good electronic conductivity, and excellent biocompatibility. The AuNP/IL-PANI inverse opaline film could be used to fabricate an electrochemical impedance spectroscopy (EIS) immunosensor for the determination of Hepatitis B surface antigen (HBsAg). The concentration of HBsAg is measured using the EIS technique by monitoring the corresponding specific binding between HBsAg and HBsAb (surface antibody). The increased electron transfer resistance (Ret) values are proportional to the logarithmic value of the concentration of HBsAg. This novel immunoassay displays a linear response range between 0.032 pg mL^-1 and 31.6 pg mL^-1 with a detection limit of 0.001 pg mL^-1. The detection of HBsAg levels in several sera showed satisfactory agreement with those using a commercial turbidimetric method.

关键词

ionic-liquid-doped polyaniline/ Inverse Opals/ Electrochemical Impedance Immunoassay